Composite

Part:BBa_K4228999:Design

Designed by: Keyao Zhao   Group: iGEM22_Tongji_China   (2022-09-29)


P43-Bromelain-T7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 312
    Illegal NheI site found at 1120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 312
    Illegal BamHI site found at 1
    Illegal XhoI site found at 646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Just for secreting protein.


Source

We chose pBE2R as our plasmid,and insert gene of bromelain to the plasmid.After reading some papers, we chose T31 to be the terminator,because it can terminates efficiently.

References